Thy1 Rat Monoclonal Antibody [Clone ID: FF-10]

Specifications

Product Data
Clone Name	FF-10
Applications	FC, IHC, IP, WB
Recommend Dilution	Flow Cytometry (1/50-1/100). Immunohistochemistry on Frozen Sections. Immunoprecipitation. Western Blot.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	EL-4 cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells from the mouse SP2/0 - Ag14 myeloma cell line.
Specificity	This antibody recognises the Thy1.2 cell surface antigen, and binds to T cell lines (EL-4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody. This clone recognises a 30kD glycoprotein in Immunoprecipitation and Western blotting assays. The antibody strongly induces homotypic adhesion of Thy1.2 positive cell lines.
Isotype	IgG1
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	thymus cell antigen 1, theta
Database Link	Entrez Gene 21838 Mouse UniProt ID P01831
Background	CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.
Synonyms	Thy-1, THY1, CDw90
Reference Data

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