MHC Class I (RT1Aa) Mouse Monoclonal Antibody [Clone ID: F16-4-4]

Specifications

Product Data
Clone Name	F16-4-4
Applications	FC, IHC
Recommend Dilution	Flow Cytometry (1/100-1/200). Immunohistochemistry on Frozen Sections.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Partially purified rat MHC antigens. Spleen cells of immunised BALB/c mice were fused with cells from the NS-1 mouse myeloma line.
Specificity	This antibody recognises the RT1A MHC class I antigen binding to a monomorphic epitope expressed by all strains, although strains of haplotype c more weakly.
Isotype	IgG1
Formulation	State: Purified State: Liquid purified IgG containing 0.09% Sodium Azide as preservative.
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P16391
Background	MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
Reference Data

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