MADCAM1 Mouse Monoclonal Antibody [Clone ID: 314G8]

Specifications

Product Data
Clone Name	314G8
Applications	ELISA, FC
Recommend Dilution	Flow Cytometry: (Neat-1/10): Use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl. ELISA: 1-10 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant soluble MAdCAM-1-fc fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
Specificity	This antibody recognises mucosal addressin cell adhesion molecule 1 (MAdCAM-1). Clone 314G8 does not recognise MAdCAM-1 in the gut lamina propria. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7. We recommend the use of SM2237LE for this purpose.
Isotype	IgG1
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin. Label: Biotin State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Biotin
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	mucosal vascular addressin cell adhesion molecule 1
Database Link	Entrez Gene 8174 Human UniProt ID Q13477
Background	MAdCAM-1 is a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria.
Synonyms	hMAdCAM-1, MAdCAM-1, Mucosal addressin cell adhesion molecule 1
Reference Data

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