Icam1 Mouse Monoclonal Antibody [Clone ID: 1A29]

Specifications

Product Data
Clone Name	1A29
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat Ax cells (a rat HEV derived cell line).
Specificity	This antibody recognizes the CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1). Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling. Functionally 1A29 inhibits homotypic aggregation of PHA blasts. We recommend SM286LE for this use.
Isotype	IgG1
Formulation	PBS, pH 7.2, containing 1% BSA as stabiliser and 0.09% Sodium Azide as preservative. Label: FITC State: Liquid purified IgG fraction Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	intercellular adhesion molecule 1
Database Link	Entrez Gene 25464 Rat UniProt ID Q00238
Background	CD54 cell surface antigen, also known as the intercellular adhesion molecule-1 (ICAM-1), is a 90 kD adhesion molecule belonging to the immunoglobulin superfamily. This is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system.
Synonyms	ICAM-1
Reference Data

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