Bromodeoxyuridine / BrDU Mouse Monoclonal Antibody [Clone ID: MoBu-1]

Specifications

Product Data
Clone Name	MoBu-1
Applications	FC, IF, IHC
Recommend Dilution	Flow Cytometry: 1-2 µg/ml. Immunocytochemistry: 2 µg/ml. Immunohistochemistry on Frozen and Paraffin Sections.
Host	Mouse
Clonality	Monoclonal
Immunogen	5-bromodeoxyuridine conjugated with hemocyanine
Specificity	The antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. The antibody MoBu-1 is also useful for detecting proliferating cells by Flow Cytometry or Immunofluorescence staining. It reacts also specifically with 5-Bromouridine (BrU).
Isotype	IgG1
Formulation	PBS, pH 7.4, with 15 mM Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction (> 95% pure by PAGE)
Concentration	lot specific
Purification	Protein A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.
Reference Data

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