HLAE (HLA-E) Mouse Monoclonal Antibody [Clone ID: MEM-E/02]

Specifications

Product Data
Clone Name	MEM-E/02
Applications	IHC, WB
Recommend Dilution	Western blotting. Immunohistochemistry on Paraffin Sections: 5-10 µg/ml. Heat antigen retrieval (Sodium Citrate). Positive Control: Human placenta. Incubation Time: 1 hour at RT (detection DAB).
Reactivity	Human, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human HLA-E denatured heavy chain
Specificity	The antibody specifically reacts with denaturated heavy chain of Human HLA-E.
Isotype	IgG1
Formulation	Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative. State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration	lot specific
Purification	Affinity Chromatography on Protein-A.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	major histocompatibility complex, class I, E
Database Link	Entrez Gene 3133 Human UniProt ID P13747
Background	HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of all human cell types. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.
Synonyms	HLA-6.2, HLAE, MHC class I antigen E
Reference Data

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