SIT (SIT1) Mouse Monoclonal Antibody [Clone ID: SIT-01]

Specifications

Product Data
Clone Name	SIT-01
Applications	FC, IP, WB
Recommend Dilution	Flow Cytometry: 1-5 μg/ml (intracellular staining). Immunoprecipitation. Western Blot: 1-2 μg/ml, reducing conditions. Western blot analysis demonstrated that SIT is expressed as an approximately 40 kDa protein that is reduced to approximately 20 kDa by endoglycosidase treatment.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Bacterially produced recombinant intracellular fragment of human SIT
Specificity	The antibody reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs.
Isotype	IgG1
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography; purity: > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	signaling threshold regulating transmembrane adaptor 1
Database Link	Entrez Gene 27240 Human UniProt ID Q9Y3P8
Background	SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
Synonyms	gp30/40; MGC125908; MGC125909; MGC125910; RP11-331F9.5; SIT
Reference Data
Protein Families	Transmembrane

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