T Cell Receptor (TCR) alpha/beta Mouse Monoclonal Antibody [Clone ID: R73]

Specifications

Product Data
Clone Name	R73
Applications	FC, FN, IHC, IP
Recommend Dilution	Functional Assays. Immunoprecipitation. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 100 µl of whole blood or 106 cells in 100 µl. Immunohistochemistry on Frozen Sections: 1/10-1/100.
Reactivity	Monkey, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat T Blasts and Erythrocytes. Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Specificity	This antibody recognizes a constant determinant on the beta chain of the Alpha/Beta T Cell Receptor, expressed by 97% of peripheral T cells as defined by the OX-52 marker. Clone R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative. This Clone is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colic et al. 2010).
Isotype	IgG1
Formulation	PBS, pH 7.4 State: Low Endotoxin State: Liquid purified IgG fraction from Tissue Culture Supernatant Stabilizer: None Preservative: None
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Synonyms	TCRA, TCRB, T-Cell Receptor alpha, T-Cell Receptor beta, T-Cell Receptor alpha beta
Reference Data

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