GST-Tag Mouse Monoclonal Antibody [Clone ID: 1E5]

Specifications

Product Data
Clone Name	100000
Applications	ELISA, ICC/IF, IF, WB
Recommend Dilution	ELISA. Western blot (1/500-1/2,000), recommended starting dilution is 1/1,000. Immunofluorescence/Immnunocytochemistry.
Reactivity	Schistosoma japonicum
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Schistosoma japonicum Glutathione S transferase (GST) purified from E. coli
Specificity	This antibody reacting specifically with GST will be useful in various immunotechniques to identify the expression of a GST fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a GST fusion protein expressing vectors.
Isotype	IgG2b, kappa
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration	1mg/ml (determined by BCA assay)
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Database Link	AAB59203.1 UniProt ID P08515
Background	Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GST proteins can be purified by immobilized glutathione affinity chromatography. Many recombinant proteins have been engineered with GST tags to facilitate the detection, isolation and purification of these proteins. Monoclonal antibody reacting specifically with GST may be useful in various immunotechnique, to identify the expression of a GST fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a GST fusion protein expressing vectors.
Synonyms	Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST
Reference Data

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