CD44 Mouse Monoclonal Antibody [Clone ID: W4-86]

Specifications

Product Data
Clone Name	W4-86
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/25-1/200 diluted antibody to label 106 cells in 100 µl. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
Reactivity	Rabbit
Host	Mouse
Clonality	Monoclonal
Immunogen	RL-5 T cell line glycoproteins. Spleen cells from an immunised mouse were fused with cells of the mouse P3.X63 Ag8 myeloma cell line.
Specificity	This antibody recognises the CD44 cell surface antigen, a 90kD glycoprotein expressed by all leucocytes. In immunohistochemical staining the antibody labels the medullary area strongly and the cortical area weakly. Reactivity in Flow cytometry may be weak. Clone W4/86 has been reported as being suitable for use in Western blotting under non-reducing conditions.
Isotype	IgG1
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD44 molecule (Indian blood group)
Database Link	UniProt ID P16070
Background	CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
Synonyms	LHR, MDU2, MDU3, MIC4, CDw44, Epican, ECMR-III, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, PGP-1
Reference Data

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