Bcl2 Binding component 3 (BBC3) alpha isoform Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 2-4 µg/ml. Detects a band of approximately 23kDa in K562 cell lysates. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Antigen retrieval using heat treatment prior to staining of paraffin sections is recommended (Sodium citrate buffer pH6.0).  Recommended Positive Control: Human breast carcinoma.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to the N-terminus of Human PUMA alpha.
Specificity	This antibody recognises the p53 - upregulated modulator of apoptosis (PUMA), also known as Bcl-2 binding component 3.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	BCL2 binding component 3
Database Link	Entrez Gene 27113 Human UniProt ID Q96PG8
Background	PUMA is a proapoptotic member of the BCL-2 family. PUMA is localised to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53.
Synonyms	BBC3, JFY-1
Reference Data

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