GFP Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IP, WB
Recommend Dilution	Immunoprecipitation: 10-20 µg/sample.  Immunocytochemistry: 1-3 µg/ml.  Western blot: 0.5-1.5 µg/ml.  Positive Control: transfected cells.  Negative Control: non-transfected cells.
Reactivity	All Species
Host	Rabbit
Clonality	Polyclonal
Immunogen	EGFP, a native full-length protein
Specificity	The antibody reacts specifically with GFP, EGFP, EYFP fusion proteins in all species.
Formulation	PBS State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. Do Not Freeze. Avoid repeated freezing and thawing.
Database Link	UniProt ID P42212
Background	Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (509 nm) when excited (excitation by Blue or UV light, absorption peak at 395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chromophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.
Synonyms	Green fluorescent protein, GFP-Tag
Reference Data

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