3-mC Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	Dot
Recommend Dilution	Dot blotting (1:400)
Reactivity	Human, Mouse, Broad
Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen for anti-3-mC antibody: 3-methylcytosine (3-mC) conjugated to BSA.
Isotype	IgG
Concentration	lot specific
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Background	3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein.
Reference Data

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