LC3B (MAP1LC3B) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, ELISA, FC, ICC/IF, IHC, Immunoblotting, IP, SDS-PAGE, Simple Western, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:200, Immunohistochemistry-Frozen, Simple Western: 1:50, Flow Cytometry, Proximity Ligation Assay, Knockdown Validated, SDS-Page, ELISA, Immunoprecipitation: 20 ug/500 ug of protein, Immunohistochemistry: 1:200 - 1:400, Immunohistochemistry-Paraffin: 1:200 - 1:400, Western Blot: 0.5 - 2.0 ug/mL, Chromatin Immunoprecipitation (ChIP), Immunoblotting, Knockout Validated
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100).
Isotype	IgG
Formulation	PBS, 30% Glycerol and 0.1% sodium azide
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	microtubule associated protein 1 light chain 3 beta
Database Link	NP_073729 Entrez Gene 67443 MouseEntrez Gene 81631 Human UniProt ID Q9GZQ8
Background	LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.
Synonyms	1BLC3; ATG8F; LC3B; MAP1A; MAP1LC3B-a
Reference Data

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