NANOG Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF, IHC, WB
Recommend Dilution	WB: 1:1000, IHC: 1:10~50, IF: 1:10~50, FC: 1:10~50
Reactivity	Human, Bovine, Monkey
Host	Rabbit
Clonality	Polyclonal
Immunogen	This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-123 amino acids from the Central region of human NANOG.
Isotype	Ig
Formulation	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	lot specific
Purification	This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	34489 Da
Gene Name	Nanog homeobox
Database Link	NP_079141 Entrez Gene 715746 MonkeyEntrez Gene 79923 Human UniProt ID Q9H9S0
Background	NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Synonyms	homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox
Reference Data
Protein Families	Cancer stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Stem cell - Pluripotency

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.