ITCH Goat Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	ELISA: 1:32,000. WB: 0.03-0.1µg/ml.
Reactivity	Human, Mouse, Rat, Dog
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.
Isotype	IgG
Formulation	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration	lot specific
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	102310 Da
Gene Name	itchy E3 ubiquitin protein ligase
Database Link	NP_113671 Entrez Gene 16396 MouseEntrez Gene 311567 RatEntrez Gene 477199 DogEntrez Gene 83737 Human UniProt ID Q96J02
Background	Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells. [provided by RefSeq]
Synonyms	ADMFD; AIF4; AIP4; dJ468O1.1; NAPP1
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Endocytosis, Ubiquitin mediated proteolysis

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