VPS25 Goat Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	ELISA: 1:1,000. WB: 0.5-1.5 µg/ml. IHC: 10µg/ml.
Reactivity	Human, Mouse, Rat, Dog
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-QPNVDTRQKQ, from the internal region of the protein sequence according to NP_115729.1.
Isotype	IgG
Formulation	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration	lot specific
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	vacuolar protein sorting 25 homolog
Database Link	NP_115729 Entrez Gene 28084 MouseEntrez Gene 681059 RatEntrez Gene 612774 DogEntrez Gene 84313 Human UniProt ID Q9BRG1
Background	VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]). [supplied by OMIM]
Synonyms	DERP9; EAP20; FAP20
Reference Data
Protein Families	Transcription Factors
Protein Pathways	Endocytosis

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