Rpe65 Goat Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	ELISA: 1:32,000. WB: 0.5-1.5µg/ml.
Reactivity	Rat, Human, Dog
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-EVKKNARKAPQPE, from the internal region of the protein sequence according to NP_000320.1.
Isotype	IgG
Formulation	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration	lot specific
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	retinal pigment epithelium-specific protein 65
Database Link	NP_446014 Entrez Gene 6121 HumanEntrez Gene 403803 DogEntrez Gene 89826 Rat UniProt ID O70276
Background	This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa. [provided by RefSeq]
Synonyms	LCA2; mRPE65; p63; rd12; RP20; sRPE65
Reference Data

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