CXCR4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	WB: 1-2 µg/mL IHC-P/IF: 5-20 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples Immunofluorescence in the human, mouse and rat samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-CXCR4 antibody (1012) was raised against a peptide corresponding to 15 amino acids near the center of human CXCR4 isoform b. The immunogen is located within amino acids 170-220 of CXCR4.
Isotype	IgG
Formulation	CXCR4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Purification	CXCR4 Antibody is Protein A purified.
Conjugation	Unconjugated
Storage Condition	CXCR4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Predicted Protein Size	Predicted: 40kD Observed: 44kD (Post-modification: 2 N-linked glycosylation)
Gene Name	C-X-C motif chemokine receptor 4
Database Link	NP_003458 Entrez Gene 7852 Human UniProt ID P61073
Background	CXCR4, a G-protein coupled receptor (GPCR) with seven transmembrane domains, is a CXC chemokine receptor specific for stromal-derived-factor-1 (SDF-1 or CXCL12). CXCR4 was initially discovered as one of the co-receptors for HIV entry into CD4+ T cells (1). Blocking CXCR4 could be potentially used as novel therapeutics for HIV treatment. CXCR4 signaling plays an important role in the migration, proliferation and quiescence of hematopoietic stem cell and their retention within the bone marrow, where it has high levels of SDF-1/CXCL12(2). It has been demonstrated that CXCR4 signaling mediates CD20 up-regulation on B cells (3). CXCR4 is highly expressed in more than 23 types of cancer, including breast cancer, ovarian cancer, melanoma, and prostate cancer, while there is very less or no expression of CXCR4 in healthy tissues. CXCR4 expression in cancer cells has been reported to be associated with tumor survival, growth and metastasis in tissues with high levels of SDF-1/CXCL12, such as lungs, liver and bone marrow (4,5). CXCR4 has been shown to regulate neuronal migration, cell positioning and axon wiring (6,7). CXCR4 mutant mice displayed aberrant neuronal distribution, which implicates the role in neuronal disorders such as epilepsy. CXCR4 is also involved in WHIM syndrome (8). WHIM mutations in CXCR4 were recently found in patients with Waldenstrom's macroglobulinemia, and these mutations are correlated to clinical resistance to ibrutinib (9,10).
Synonyms	CD184; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM
Note	Optimal dilutions for each application to be determined by the researcher.
Reference Data

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