TRIM5 alpha (TRIM5) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 2 ug/mL, ICC: 1 ug/mL, IF: 10 ug/mL
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	TRIM5 delta antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy-terminus of human TRIM5 delta.
Isotype	IgG
Formulation	PBS containing 0.02% sodium azide.
Concentration	1ug/ul
Purification	Affinity chromatography purified via peptide column
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	tripartite motif containing 5
Database Link	NP_149084 Entrez Gene 85363 Human UniProt ID Q9C035
Background	TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region (1). TRIM5 has five alternately spliced isoforms, the longest of which is the ? variant which also contains a carboxy-terminal B30.2 (SPRY) domain (1). Expression of TRIM5alpha variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1 (2,3), albeit at differing efficiencies. The TRIM5delta isoform has been shown to colocalize with the topoisomerase I-interacting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5delta also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner (4).
Synonyms	RNF88; TRIM5alpha
Reference Data

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