UBE2N Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	UBC13 antibody can be used for detection of UBC13 by Western blot at 0.5 to 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 10 µg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	UBC13 antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human UBC13.
Isotype	IgG
Formulation	PBS containing 0.02% sodium azide.
Concentration	1ug/ul
Purification	UBC13 Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	ubiquitin conjugating enzyme E2 N
Database Link	AAP35519 Entrez Gene 7334 Human UniProt ID P61088
Background	Ubiquitin-conjugating enzyme 13 (Ubc13) was initially discovered in S. cerevisiae as a DNA-damage inducible protein involved in the error-free DNA postreplication repair pathway (1). It has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway (reviewed in 2). Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors (2,3). Ubc13 is part of a dimeric ubiquitin-conjugating enzyme complex also containing UEV1A (ubiquitin-conjugating enzyme E2 variant 1) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family (4-6). The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation (5).
Synonyms	HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN
Reference Data

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