GLI3 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	ELISA: 1:6,000 - 1:30,000, WB: 1:500 - 1:2,000, IHC: 0.5 mg/ml - 5 ug/ml
Reactivity	Human, Chimpanzee, Squirrel Monkey, Xenopus, Chicken, Dog
Host	Rabbit
Clonality	Polyclonal
Immunogen	This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to amino acids 41-57 of human Gli-3 protein.
Isotype	IgG
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	GLI family zinc finger 3
Database Link	NP_000159 Entrez Gene 483244 DogEntrez Gene 702494 MonkeyEntrez Gene 2737 Human UniProt ID P10071
Synonyms	ACLS; GCPS; GLI3-190; GLI3FL; PAP-A; PAPA; PAPA1; PAPB; PHS; PPDIV
Note	Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder-affecting limb and cranio-facial development. Two isoforms of human Gli-3 have been reported. One is the full-length protein at ~170-190kDa and the other is a truncated isoform at ~80kDa.
Reference Data
Protein Families	Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS
Protein Pathways	Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer

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