SIGLEC15 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1-4 µg/mL IHC-P/IF: 5-20 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples Immunofluorescence in the human, mouse and rat samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-SIGLEC15 antibody (6765) was raised against a peptide corresponding to 15 amino acid synthetic peptide near the amino terminus of human SIGLEC15. The immunogen is located within amino acids 50-100 of SIGLEC15.
Specificity	SIGLEC15 antibody is predicted to not cross-react with other SIGLEC family members.
Isotype	IgG
Formulation	SIGLEC15 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Purification	SIGLEC15 Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	SIGLEC15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Predicted Protein Size	Predicted: 36 kD Observed: 45 kD (PTM: 1 N-linked glycosylation)
Gene Name	sialic acid binding Ig like lectin 15
Database Link	NP_998767 Entrez Gene 498888 RatEntrez Gene 620235 MouseEntrez Gene 284266 Human UniProt ID Q6ZMC9
Background	SIGLEC15 Antibody: Siglecs are vertebrate cell-surface lectins that recognize sialylated glycans and are involved in many physiological processes, such as glycoprotein turnover, cellular trafficking, and pathogen recognition. Most Siglecs are expressed on cells of the immune system. SIGLEC15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain containing a lysine residue, and a short cytoplasmic tail. SIGLEC15 can interact with the activating adaptor molecules DAP12/10. Its activating signaling potential and unique preference for glycan recognition implies that SIGLEC15 may be involved in the immune surveillance of tumors and probably plays a conserved, regulatory role in the immune system of vertebrates.
Synonyms	CD33L3; HsT1361; SIGLEC-15
Note	Optimal dilutions for each application to be determined by the researcher.
Reference Data
Protein Families	Transmembrane

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