Dlg1 Mouse Monoclonal Antibody [Clone ID: S64-15]

Specifications

Product Data
Clone Name	S64-15
Applications	IHC, WB
Recommend Dilution	WB: 1:1000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Fusion protein amino acids 1-104 of rat SAP97
Formulation	PBS pH7.4, 50% glycerol
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	discs large homolog 1, scribble cell polarity complex component
Database Link	NP_036920 Entrez Gene 1739 HumanEntrez Gene 13383 MouseEntrez Gene 25252 Rat UniProt ID Q62696
Background	Synapse-Associated Protein 97 (SAP97/human homologue of Drosophila discs-large tumor supressor or HDLG) is one of a family of plasma membrane-associated proteins found in synaptic junctions. This is a novel, presynaptic membrane protein homologous to SAP90 and the Drosophila discs-large tumor supressor protein. SAP97 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity . SAP97 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and Shaker-type potassium channels at the synaptic membrane. There are two principal modes ofinteraction between SAP97 and other proteins. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif . Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP97 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP97 in vitro through a pseudo-homotypic PDZ-PDZ interaction .
Synonyms	dJ1061C18.1.1; DKFZp761P0818; DKFZp781B0426; DLGH1; hdlg; OTTHUMP00000165203; SAP-97; SAP97
Note	Detects ~130kDa. No cross-reactivity against PSD95, SAP102 and Chapsyn110 expressed in transfected cells.
Reference Data

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