TRPV4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	WB: 0.5-2 µg/mL IHC-P: 1-2 µg/mL IHC-P/IF: 5-20 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples Immunohistochemistry in human, mouse, and rat samples Immunofluorescence in the human sample. All other applications and species not yet tested.
Reactivity	Human, Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-TRPV4 antibody (7695) was raised against a peptide corresponding to 18 amino acid synthetic peptide near the center of human TRPV4. The immunogen is located within amino acids 380-430 of TRPV4.
Specificity	TRPV4 antibody is human specific. At least six isoforms of TRPV4 are known to exist. This antibody is predicted to not cross-react with other TRP protein family members.
Isotype	IgG
Formulation	TRPV4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Purification	TRPV4 antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	TRPV4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Predicted Protein Size	Predicted: 98kD Observed: 98 kD
Gene Name	transient receptor potential cation channel subfamily V member 4
Database Link	NP_067638 Entrez Gene 59341 Human UniProt ID Q9HBA0
Background	The transient receptor potential (TRP) protein family consists of a diverse group of cation channels functioning in a variety of homeostatic and regulatory pathways. Four subfamilies exist, based on channel domain homology: C type (canonical), V type (vanilloid receptor related), M type (melastatin related) and P type (PKD) (1). TRPV4, belongs to the V type subfamily and plays a role in systemic osmoregulation (2,3). TRPV4 is a calcium channel multi-pass membrane protein activated by various stimuli, including thermal stress, fatty acid metabolites and hypotonicity (3). TRPV4 is highly expressed in lung and kidney and widely expressed in brain. It plays an important role in regulating neural excitability (4).
Synonyms	BCYM3; CMT2C; HMSN2C; OTRPC4; SMAL; SPSMA; SSQTL1; TRP12; VRL2; VROAC
Note	Optimal dilutions for each application to be determined by the researcher.
Reference Data
Protein Families	Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane

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