KCNK2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:200-1:2000
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide DPKSAAQNSKPRLSFSTK(C), corresponding to residues 8-25 of human K2P2.1 (TREK-1). Intracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	potassium two pore domain channel subfamily K member 2
Database Link	NP_001017424 Entrez Gene 170899 Rat UniProt ID O95069
Background	K2P2.1 (also named TWIK-Related K+ channel, TREK-1 or KCNK2) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time or voltage dependence and are considered to be â??leakâ? or â??backgroundâ? K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. K2P2.1 can be integrated to a K2P subfamily that includes K2P4.1 (TRAAK) and K2P10.1 (TREK2) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, lysophosphatidic acid and mechanical stretch. In addition, K2P2.1 can also be activated by general anesthetics such as halothane and chloroform and intracellular acidification. K2P2.1 expression in humans is largely restricted to the brain with some expression in ovary and small intestine while K2P2.1expression in rodents is more widespread. K2P2.1 has an important role in mood regulation, as knockout mice show resistance to depression, suggesting that K2P2.1 may be a potential target for anti-depressants. K2P2.1 is involved in the fast release of glutamate from astrocytes. It requires the activation of Gai, dissociation of GÃ??, followed by the opening of the glutamate permeable K2P2.1 (TREK-1) K+ channel through its interaction with GÃ??, For this purpose, TREK-1 is localized at the cell surface of the cell body and processes of astrocytes.
Synonyms	hTREK-1c; hTREK-1e; K2p2.1; TPKC1; TREK; TREK-1; TREK1
Reference Data
Protein Families	Druggable Genome, Ion Channels: Potassium, Transmembrane

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