Gfra1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide CRSAMEALKQKS, corresponding to amino acid residues 72-83 of rat GFRa1. Extracellular domain.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	GDNF family receptor alpha 1
Database Link	NP_037091 Entrez Gene 2674 HumanEntrez Gene 14585 MouseEntrez Gene 25454 Rat UniProt ID Q62997
Background	The GDNF family ligands (GFLs) belong to the super family of the TGF-Ã?. They belong to the group of cystine-knot protein and function as homodimers. This family includes glial cell line-derived neurotrophic factor (GDNF), artemin (ARTN), Neurturin (NRTN) and persephin (PSPN).These factors are heavily involved in the development and function of the nervous system (both central and peripheral). In particular GDNF has an important role outside the nervous system where it plays a role in kidney morphogenesis.In general GFLs all signal through a signal through the receptor tyrosine kinase Ret. Their specificity is implemented by different GDNF family receptor a (GFRa), which act as co-receptors. These extracellular proteins are bound to the plasma membrane via a glycosyl phosphatidyl inositol (GPI) anchor. GFRa1-4 are responsible for the binding of GDNF, NRTN, ARTN, and PSPN respectively and the subsequent activation of Ret. Soluble forms of the receptor by the cleavage of a yet unknown phospholipase or protease can be detected. Also alternative spliced forms of the protein can lead to soluble forms of GFRa receptors. In respect to their important role in development, individual knockout of either gdnf, gfra1 or ret gives rise to lethal phenotypes.
Synonyms	DKFZp313E1012; DKFZp686J0156; FLJ10561; FLJ31546; GDNFR; GDNFR-alpha; GDNFRA; GFR-ALPHA-1; MGC23045; RET1L; RETL1; TRNR1
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.