Slc8a1 Rabbit Polyclonal Antibody

CAT#: TA328915

Rabbit polyclonal Anti-Na+/Ca2+Â Exchanger 1 (NCX-1)



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CNY 11,000.00


货期*
4周

规格
    • 50 ul

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Specifications

Product Data
Applications IHC, WB
Recommend Dilution WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Peptide (C)EVDERDQDDEEAR, corresponding to amino acid residues 308-320 of rat NCX-1 . 3rd intracellular loop.
Formulation Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification Affinity purified on immobilized antigen.
Conjugation Unconjugated
Storage Condition Store at -20°C as received.
Gene Name solute carrier family 8 member A1
Background Ca2+Â has proven to be a universal signaling molecule in excitable and non-excitable cells. However, being that its intracellular concentration is 1000 time lower than the extracellular milieu, it is important for the cell to keep this ratio for proper function. NCX, a Na+/Ca2+Â exchanger is responsible for most of the efflux of Ca2+Â out from the cell.The NCX transporter is a member of the SLC8 family of solute carriers which in turn belong to the CaCA superfamily.NCX-1 is one of three Na+/Ca2+ exchangers (NCX-1, NCX-2, NCX-3) leading to one Ca2+ movement across the plasma membrane in exchange of three Na+ influx. However, the transporter can reverse the direction of the transport if the concentrations of Na+ and Ca2+ change.The transporter has nine transmembrane domains and intracellular N- and C-terminals. Between tansmembrane domains 5 and 6, the presence of an extra-long intracellular loop, termed the f loop is responsible for regulating the activity of NCX-1 via several different mechanisms like ion binding, phosphorylation, etc. The f loop also has sites which undergo alternative splicing.Of the three NCX-1 expressed in mammalian cells, NCX-1 is the most widely expressed. Its expression is detected in the heart, brain, and kidney. NCX-1 undergoes alternative splicing in a tissue dependent manner. The first splice region does not change the overall structure of the protein but rather enables the expression of the gene specific to the tissues which require the expression of the gene. The second splicing site leads to a number of proteins varying in length. NCX-2 expression is much more limited; it is expressed only in neurons. NCX-3 is expressed in skeletal muscle and in some regions of the brain and undergoes alternative splicing in a similar fashion to that of NCX-1.Due to its central role in modulating Ca2+ levels in the cell, NCX-1 has become a pharmaceutical target in the development of drugs for various heart diseases and neurological disorders.
Synonyms CNC; DKFZp779F0871; FLJ37694; FLJ43417; MGC119581; NCX1; OTTHUMP00000128378; OTTHUMP00000201569; OTTHUMP00000201570; OTTHUMP00000201587; OTTHUMP00000201589
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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