IRE1 (ERN1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, ELISA, ICC/IF, IHC, Immunoblotting, IP, WB
Recommend Dilution	Western Blot: 1:500 - 1:1000, Knockdown Validated, Immunocytochemistry/ Immunofluorescence: 1:10 - 1:500, Chromatin Immunoprecipitation (ChIP), Immunoblotting, In vitro assay, Immunohistochemistry-Paraffin: 1:10 - 1:500, Immunohistochemistry-Frozen: 1:10 - 1:500, ELISA: 1:100 - 1:2000, Immunohistochemistry: 1:10 - 1:500, Immunoprecipitation: 1:10 - 1:500
Reactivity	Human, Mouse, Rat, Rabbit
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide surrounding the phosphorylated serine 724 of the human IRE1 alpha protein. [Swiss-Prot #O75460]
Formulation	PBS, 0.01% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	endoplasmic reticulum to nucleus signaling 1
Database Link	NP_001424 Entrez Gene 78943 MouseEntrez Gene 498013 RatEntrez Gene 2081 Human UniProt ID O75460
Background	IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. It is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities. mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER.
Synonyms	hIRE1p; IRE1; IRE1a; IRE1P
Note	This IRE1 pS724 antibody is useful for Western blot, ELISA, and Immunohistochemistry paraffin embedded sections.(PMID: 19264902) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24823368)
Reference Data
Protein Families	Protein Kinase, Transmembrane
Protein Pathways	Alzheimer's disease

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