Caspase 9 (CASP9) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, IP, WB
Recommend Dilution	Western Blot, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin: 1:1000-1:5000, Immunohistochemistry-Frozen: Assay Dependent, Flow (Intracellular), Immunoprecipitation: 1:50-1:200
Reactivity	Human, Mouse, Rat, Canine, Gerbil
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant catalytically active human Caspase-9 protein was used as immunogen (NP_001220).
Formulation	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	caspase 9
Database Link	NP_001220 Entrez Gene 12371 MouseEntrez Gene 58918 RatEntrez Gene 842 Human UniProt ID P55211
Background	Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
Synonyms	APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56
Note	This antibody is useful for immunoprecipitation, Western Blot, Immunohistochemistry-Paraffin and Immunohistochemistry-Frozen . Use in Flow Intracellular reported in scientific literature (PMID 24804954)
Reference Data
Protein Families	Druggable Genome, Protease, Stem cell - Pluripotency
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Endometrial cancer, Huntington's disease, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Parkinson's disease, Pathways in cancer, Prostate cancer, Small cell lung cancer, VEGF signaling pathway, Viral myocarditis

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