NF-kB p65 (RELA) Mouse Monoclonal Antibody [Clone ID: 112A1021]

Specifications

Product Data
Clone Name	112A1021
Applications	CyTOF-ready, FC, IHC, IP, Simple Western, WB
Recommend Dilution	Immunohistochemistry: 5 - 15 ug/mL, Western Blot: 2 - 5 ug/mL, Flow (Intracellular)), Immunohistochemistry-Paraffin: 5 ug/mL, Simple Western: 1:25, Immunoprecipitation, Flow Cytometry: 0.25 - 0.5 ug/10^6 cells, CyTOF-ready
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody was raised against a synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	RELA proto-oncogene, NF-kB subunit
Database Link	NP_068810 Entrez Gene 19697 MouseEntrez Gene 309165 RatEntrez Gene 5970 Human UniProt ID Q04206
Background	NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.
Synonyms	NFKB3; p65
Note	Western Blot and Flow cytometry/analysis (Intracellular) as reported in the literature (Wittmann et al, 2005). IHC (paraffin) as reported in the literature (Chang et al, 2005)
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway

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