TIGAR Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Immunohistochemistry: 1:10-1:500, Western Blot: 1:5000, Immunohistochemistry-Paraffin: 1:10-1:500
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A genomic peptide made to an internal region of the human TIGAR protein (within residues 50-200). [Swiss-Prot Q9NQ88]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	TP53 induced glycolysis regulatory phosphatase
Database Link	NP_065108 Entrez Gene 319801 MouseEntrez Gene 57103 Human UniProt ID Q9NQ88
Background	TIGAR (TP53-induced glycolysis and apoptosis regulator), a member of phosphoglycerate mutase family, is a target gene of p53 and it regulates glucose metabolism in cancer cells by acting as a fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate (Fru-2,6-P2 ) and Fru-1,6-P2 leading to decreased Fru-2,6-P2 levels with subsequent reduction in PFK1 (phosphofructosekinase-1) activity as well as negative regulation of glycolysis. TIGAR also reduces cellular ROS through its translocation to the mitochondria where it interacts with HK2 leading to enhanced HK2 activity, regulation of mitochondrial membrane potential, and decreased mitochondrial ROS which ultimately protects the cells from DNA damage-induced apoptosis. Moreover, TIGAR mediated negative regulation of glycolysis leads to redirection of glycolytic metabolic intermediates towards pentose phosphate pathway's oxidative branch that follows increased cellular NADPH production, ROS scavenging by GSH and thus a lower sensitivity of cells to oxidative stress-associated apoptosis, including that induced by p53. Because of TIGAR's anti-apoptotic/protective role, it has been proposed to implicate in cell-cycle arrest and DNA repair effector response in cells that suffer from repairable dose of genotoxic/cellular insult.
Synonyms	C12orf5; FR2BP
Note	This TIGAR antibody is useful for Immunohistochemistry-paraffin sections and Western blot. In Western blot bands are seen ~30 kDa, representing TIGAR, and ~47 kDa. At this time we cannot explain the band at 47 kDa. This antibody does not appear to work in ICC.
Reference Data

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