BNIP3L Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC
Recommend Dilution	Immunohistochemistry: 1:300, Immunocytochemistry/ Immunofluorescence: 1:100-1:1000, Immunohistochemistry-Paraffin: 1:300
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal portion of the human BNIP3L protein (between residues 50-100) [UniProt O60238]
Formulation	PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	BCL2/adenovirus E1B 19kDa interacting protein 3-like
Database Link	NP_004322 Entrez Gene 12177 MouseEntrez Gene 665 Human UniProt ID O60238
Background	BNIP3L/NIX is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family, which is involved in the regulation of apoptosis. Like other BH3-only proteins, NIX is believed to possess tumor suppressing functions; NIX localizes to the mitochondria and interacts with BCL2 and BCL-X in a pro-apoptotic manner. NIX shares a high level of sequence homology with BNIP3, and like BNIP3 activates apoptosis through its C-terminal transmembrane domain but not via its BH3 domain. Hypoxia is believed to be a principle regulator of NIX activity, making NIX a key factor in hypoxia-induced tumor cell death and heart disease. In addition, NIX has been implicated in mitophagy, a specific form of autophagy; due to its dual role, NIX is an interesting target to examine the relationship between apoptosis and autophagy.
Synonyms	BNIP3a; NIX
Note	This BNIP3L antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry-Paraffin. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, Transmembrane

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