AMPK alpha 1 (PRKAA1) Mouse Monoclonal Antibody [Clone ID: 2B7]

Specifications

Product Data
Clone Name	2B7
Applications	ELISA, FC, ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Flow Cytometry: 1:200-1:400, ELISA: 1:10000, Immunohistochemistry-Paraffin: 1:200-1:1000, Simple Western: 1:50, Western Blot: 1:500, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Immunohistochemistry: 1:200 - 1:1000
Reactivity	Human, Mouse, Rat, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human AMPK alpha 1 expressed in E. coli. [UniProt# Q13131]
Isotype	IgG1
Formulation	PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ammonium sulfate precipitation
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	64 kDa
Gene Name	protein kinase AMP-activated catalytic subunit alpha 1
Database Link	NP_996790 Entrez Gene 65248 RatEntrez Gene 105787 MouseEntrez Gene 5562 Human UniProt ID Q13131
Background	The protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Synonyms	AMPK; AMPKa1
Note	This AMPK alpha 1 (2B7) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy

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