DNA PKcs (PRKDC) Mouse Monoclonal Antibody [Clone ID: 3H6]

Specifications

Product Data
Clone Name	3H6
Applications	ELISA, ICC/IF, IHC, WB
Recommend Dilution	ELISA: 1:10000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Immunohistochemistry-Paraffin: 1:200-1:1000, Western Blot: 1:500-1:2000, Immunohistochemistry
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human DNA PKcs expressed in E. coli. [UniProt# P78527]
Isotype	IgG1
Formulation	Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ascites
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	460 kDa
Gene Name	protein kinase, DNA-activated, catalytic polypeptide
Database Link	NP_001075109 Entrez Gene 5591 Human UniProt ID P78527
Background	The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.
Synonyms	DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IMD26; p350; XRCC7
Note	This DNA PKcs (3H6) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and ELISA. In WB, a band can be seen at 460-470 kDa representing DNA PKcs.
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Cell cycle, Non-homologous end-joining

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