14-3-3 sigma (SFN) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Immunohistochemistry, Knockdown Validated, Western Blot: 3-5ug/ml~, Immunohistochemistry-Paraffin: 5-10ug/ml
Reactivity	Human, Amphibian, Bovine, Canine, Equine, Opossum
Host	Rabbit
Clonality	Polyclonal
Immunogen	A portion of amino acids 120-170 of human 14-3-3 sigma was used as the immunogen for this antibody.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein A purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	stratifin
Database Link	NP_006133 Entrez Gene 2810 Human UniProt ID P31947
Background	14-3-3 sigma (stratifin, SFN, epithelial cell marker protein 1) is a member of a highly conserved family of 14-3-3 proteins that are present in all eukaryotic organisms. There are 7 known mammalian 14-3-3 isoforms (sigma epsilon, beta, zeta, gamma, theta, tau and they play important roles in many biological activities by binding to and altering the subcellular localization and/or stability of key molecules in various signaling cascades. 14-3-3 sigma was originally characterized as a human mammary epithelium marker and later rediscovered as an important molecule for cell cycle checkpoint regulation. Recently it has been reported that 14-3-3 sigma may serve as a prognosis marker predicting survival of pancreatic cancer patient treatment. 14-3-3 sigma is the only 14-3-3 isoform induced by the tumor suppressor protein p53, in response to irradiation and other DNA-damaging agents. 14-3-3 sigma is a p53-regulated inhibitor of G2/M progression and acts as a tumor suppressor gene that is inactivated by methylation of its 5' CpG islands in epithelial tumor cells. Two isoforms of human 14-3-3 sigma are produced by alternative splicing.
Synonyms	YWHAS
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Cell cycle, p53 signaling pathway

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