HIF1AN Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 500-2000 WB positive control: Mouse brain and liver tissue IHC: 50-300 Positive control: Human esophagus cancer Predicted cell location: Cytoplasm and Nucleus
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide of human HIF1AN
Isotype	IgG
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
Concentration	lot specific
Purification	Antigen affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	40 kDa
Gene Name	hypoxia inducible factor 1 alpha subunit inhibitor
Database Link	NP_060372 Entrez Gene 319594 MouseEntrez Gene 55662 Human UniProt ID Q9NWT6
Background	Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.
Synonyms	FIH1
Reference Data
Protein Families	Druggable Genome

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