Interferon gamma (IFNG) Mouse Monoclonal Antibody [Clone ID: MD-1]

Specifications

Product Data
Clone Name	MD-1
Recommend Dilution	ELISA, ELISPOT, Immunohistochemistry
Reactivity	Human, Rhesus Macaque
Host	Mouse
Clonality	Monoclonal
Immunogen	IFN-gamma
Isotype	IgG1
Formulation	Prior to lyophilization: 0.5 ml PBS + 125 mM trehalose.
Reconstitution Method	Dissolve the contents of the vial by injection of 0.5 ml sterile distilled water.
Concentration	lot specific
Purification	Ion exchange chromatography.
Conjugation	Biotin
Storage Condition	Lyophilized product is stable for at least one year at 4°C (expiry date is indicated on the vial). After reconstitution, the contents can be safely stored at 4°C for one month or for one year at –20°C. Add 0.02% sodium azide to prevent bacterial growth.
Predicted Protein Size	16.7 kDa
Gene Name	interferon, gamma
Database Link	NP_000610 Entrez Gene 3458 Human UniProt ID P01579
Background	The monoclonal antibody to human IFN-? (clone MD-1) was first described in 1985. It has shown to neutralize the antiviral activity of both natural and recombinant human IFN-? and in a Western Blot the antibody reacts with the 20 kDa and 25 kDa polypeptides present in natural human IFN-? (van der Meide P.H. et al. 1985). The MD-1 antibody has been descriped as useful in a sandwich ELISA (Wassenaar A. et al. 1995, Wierenga E.A. et al. 1990 and Yazdanbakhsh M et al. 1993). Further, the antibody has proven to cross react with rhesus macaque IFN-? and can be used for immunohistochemical staining of frozen tissues (Wassenaar A. et al. 1995 and Kap Y. et al. 2009). The MD-1 antibody has been reported for use in intracellular flow cytometric analysis (Hamann D. et al. 1996). Other available formats: unconjugated and FITC labeled.
Synonyms	IFG; IFI
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Allograft rejection, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, Proteasome, Regulation of autophagy, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Type I diabetes mellitus

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