Ifng Mouse Monoclonal Antibody [Clone ID: DB-1]

Specifications

Product Data
Clone Name	DB-1
Recommend Dilution	ELISA, Intracellular staining, Immunohistochemistry
Reactivity	Rat, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	IFN-gamma
Isotype	IgG1
Formulation	Prior to lyophilization: 0.5 ml PBS + 1% BSA + 125 mM trehalose.
Concentration	lot specific
Purification	Ion exchange chromatography.
Conjugation	Biotin
Storage Condition	Store at -20°C as received.
Predicted Protein Size	17.9 kDa
Gene Name	interferon gamma
Database Link	NP_620235 Entrez Gene 15978 MouseEntrez Gene 25712 Rat UniProt ID P01581
Background	The monoclonal antibody to rat IFN-? (clone DB-1) was first described in 1989. It has shown to neutralize both natural and recombinant rat IFN-? and mouse IFN-? in vivo and in vitro. In a Western Blot the antibody reacts with the 14 kDa and 18 kDa polypeptides present in natural rat IFN-? (van der Meide P.H. et al. 1989). The biotinyated DB-1 antibody is highly effective as detector antibody in both sandwich ELISA and ELISPOT assays using DB-12 (cat no. CT052) as coating antibody. Further, the DB-1 antibody can be used for immunohistochemical staining of frozen tissues (Aguilar Torrentera F. et al. 2002). Other available formats: unconjugated and FITC labeled.
Synonyms	IFG; IFI; IFN-gamma
Reference Data

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