Ifng Mouse Monoclonal Antibody [Clone ID: DB-12]

Specifications

Product Data
Clone Name	DB-12
Recommend Dilution	ELISA, ELISPOT, Intracellular staining, Immunohistochemistry
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	IFN-gamma
Isotype	IgG1
Formulation	Prior to lyophilization: 0.5 ml PBS + 1% BSA + 125 mM trehalose.
Concentration	lot specific
Purification	Ion exchange chromatography.
Conjugation	Biotin
Storage Condition	Store at -20°C as received.
Predicted Protein Size	17.9 kDa
Gene Name	interferon gamma
Database Link	NP_620235 Entrez Gene 25712 Rat UniProt ID P01581
Background	The mouse anti-rat IFN-? monoclonal antibody (clone DB-12) was first described by Van der Meide P.H. et al. in 1989. The rat IFN-? antibody is of the IgG1 subtype and possesses very low neutralizing activity. In Western blot analysis the DB-12 antibody reacts with the 14 kDa and 18 kDa polypeptides present in rat IFN-? with moderate activity and it has shown not to bind with either mouse IFN-? nor human IFN-?. Further, the DB-12 antibody is highly effective as coating antibody in both sandwich ELISA and ELISPOT assays using biotinylated DB-1 antibody for detection. Also the reverse combination in which the DB-1 antibody functions as coating antibody and biotinylated DB-12 antibody as detector can be used in an ELISA system (Haagmans B.L. et al. 1994). The DB-12 antibody is also applicable in immunohistochemistry on frozen tissues and intracellular staining (Nennesmo, I. et al. 1989 and Bernard, I et al. 1998).
Synonyms	IFG; IFI; IFN-gamma
Reference Data

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