TIM 3 (HAVCR2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1-2µg/mL. IHC: 2µg/mL. Immunoflorescence start at 20µg/mL.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	TIM3 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM3. The immunogen is located within amino acids 60 - 110 of TIM3.
Formulation	TIM3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	TIM3 Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 33 kDa; Observed: 37 kDa
Gene Name	hepatitis A virus cellular receptor 2
Database Link	NP_116171 Entrez Gene 84868 Human UniProt ID Q8TDQ0
Background	The immune checkpoint protein TIM3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-? producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).
Synonyms	FLJ14428; HAVcr-2; KIM-3; Tim-3; TIM3; TIMD-3; TIMD3
Note	TIM3 antibody can be used for Western blot at 1 - 2 µg/mL. Antibody can also be used for Immunohistochemistry at 2 µg/mL. For Immunoflorescence start at 20 µg/mL.
Reference Data

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