B7-1 (CD80) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	WB: 1-4 µg/mL IHC-P/IF: 5-20 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples Immunofluorescence in the human, mouse and rat samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-CD80 antibody (8679) was raised against a peptide corresponding to 17 amino acid synthetic peptide near the amino terminus of human CD80. The immunogen is located within amino acids 60-110 of CD80.
Isotype	IgG
Formulation	CD80 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Purification	CD80 Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	CD80 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Predicted Protein Size	Predicted: 60 kD Observed: 60 kD
Gene Name	CD80 molecule
Database Link	NP_005182 Entrez Gene 941 Human UniProt ID P33681
Background	CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
Synonyms	B7; BB1; CD28LG; CD28LG1; LAB7
Note	Optimal dilutions for each application to be determined by the researcher.
Reference Data

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