B7H3 (CD276) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 0.5-1µg/mL.IHC: 2µg/mL. Immunoflorescence start at 20µg/mL.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	CD276 antibody was raised against a peptide corresponding to 19 amino acids near the carboxy terminus of CD276. The immunogen is located within amino acids 484 - 534 of CD276.
Formulation	CD276 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	CD276 Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 35, 43, 57 kDa; Observed: 59 kDa
Gene Name	CD276 molecule
Database Link	NP_001019907 Entrez Gene 80381 Human UniProt ID Q5ZPR3
Background	CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.
Synonyms	4Ig-B7-H3; B7-H3; B7H3
Note	CD276 antibody can be used for Western blot at 0.5 - 1 µg/mL. Antibody can also be used for Immunohistochemistry at 2 µg/mL. For Immunoflorescence start at 20 µg/mL.
Reference Data

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