Rhodopsin (RHO) Mouse Monoclonal Antibody [Clone ID: Rho 1D4]

Specifications

Product Data
Clone Name	Rho 1D4
Applications	ELISA, IF, IHC, IP, WB
Reactivity	Mouse, Rat, Cow, Human, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Bleached bovine rod outer segment (ROS).
Specificity	The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold. The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein-coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag.
Isotype	IgG1, kappa
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	rhodopsin
Database Link	Entrez Gene 6010 Human UniProt ID P08100
Synonyms	CSNBAD1; MGC138309; MGC138311; OPN2; Opsin-2; rhodopsin; RP4
Note	This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
Reference Data

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