Ctla4 Rabbit Monoclonal Antibody [Clone ID: 9D9]

Specifications

Product Data
Clone Name	9D9
Applications	Bl, IF
Reactivity	Mouse
Host	Rabbit
Clonality	Monoclonal
Immunogen	The antibody was raised against CTLA-4.
Specificity	The antibody is specific for CTLA-4. The antibody has been used to block CTLA-4 and has been shown to enhance T-cell immunity against tumours (Quezada et al, 2006). 9D9 also has an anti-B16/BL6 melanoma effect in non-irradiated tumour-bearing recipients when given as combinatorial therapy with Gvax (Peggs et al, 2009). Selby et al. (PMID: 24777248) show how conversion of the original mouse IgG2b to a mouse IgG2a greatly increases anti-tumor activity of the 9D9 antibody in both MC38 (C57BL/6 mice) and CT26 (BALB/c mice) models. This mechanism both involves blockade of CTLA4 as well as depletion of tumor-infiltrating regulatory T-cells. Fc Silent antibodies were shown to have reduced anti-tumor activity as they did not deplete tumor infiltrating regulatory T-cells, however they lead to expansion of peripheral Tregs.
Isotype	IgG, kappa
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	cytotoxic T-lymphocyte-associated protein 4
Database Link	Entrez Gene 12477 Mouse UniProt ID P09793
Synonyms	CD; CD28; CD152; CELIAC3; CTLA-4; GRD4; GSE; ICOS; IDDM12
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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