CD137 (TNFRSF9) Rabbit Monoclonal Antibody [Clone ID: 4B4-1-1]

Specifications

Product Data
Clone Name	4B4-1-1
Applications	Bl, ELISA, FC, IHC
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	The original antibody was generated by immunizing BALB/c mice with a fusion protein of human 4-1BB antigen and glutathione s transferase (GST).
Specificity	This antibody binds to the human 4-1BB, which is expressed on the surface of activated T cells. The antibody has good binding affinity for Con A-activated T cells and has an immunosuppressive activity towards lymphocyte proliferation in the in vitro mixed lymphocyte reaction. The binding of antibody to surface expressed 4-1BB molecule was assessed using flow cytometry and ELISA was used to check whether the cell culture had binding affinity to 4-1BB (Kim et al., 1995). The interaction of this antibody with CD137 is reported to induce an increase in TNF-α production by monocytes. This antibody competes with mAb M121 for binding to CD137 (PMID: 10607752). This antiboy also induces the production of IFN-γ in NK cells in presence of IL-15 (PMID: 20008791).This antibody can be used in blocking assays and staining methods (PMID: 14746806). Humanized versions of this antibody (Hz4B4-1) and (Hz4B4-2) showed a 1.5 fold and 7.3 fold increase and in binding affinity for 4-1BB as compared to their parental mouse antibody. Additionally, H4B4 mediated antibody-dependent cellular toxicity of activated human peripheral blood T cells and CEM cells in a dose dependent manner (PMID: 11186149). Humanized version of this antibody is available on request.
Isotype	IgG, kappa
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	tumor necrosis factor receptor superfamily member 9
Database Link	Entrez Gene 3604 Human UniProt ID Q07011
Synonyms	4-1BB; CD137; CDw137; ILA; MGC2172
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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