AGO2 Rabbit Polyclonal Antibody

CAT#: TA387389

AGO2 Rabbit polyclonal antibody



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CNY 1,999.00

CNY 3,280.00


货期*
2周

规格
    • 100 ug

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经常一起买 (1)
beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
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Specifications

Product Data
Applications ChIP, IHC, WB
Recommend Dilution Recommended dilution: WB:1:1000-1:5000, IHC:1:100-1:1500
Reactivity Mouse, Human
Host Rabbit
Clonality Polyclonal
Immunogen Recombinant Human Protein argonaute-2 protein (517-818AA)
Formulation Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Concentration lot specific
Purification >95%, Protein G purified
Conjugation Unconjugated
Storage Condition Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Background Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The minimal RISC appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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