Fas Ligand (FASLG) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:1000~1:2000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant protein of human CD178.
Specificity	CD178 polyclonal antibody detects endogenous levels of CD178 protein.
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 45 kDa
Gene Name	Fas ligand
Database Link	Entrez Gene 356 Human UniProt ID P48023
Background	Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers. Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes. FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40 kDa upon glycosylation) that belongs to the TNF family, which also includes TNF-α, TRAIL, and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8. Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites. FasL is also expressed in several tumor types as a mechanism to evade immune surveillance. Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26 kDa trimeric soluble form.
Synonyms	ADAM10-processed FasL form; APL; Apoptosis antigen ligand; APT1LG1; APTL; CD95 ligand; CD95-L; CD95L; CD178; Fas antigen ligand; FASL; FasL; FASLG; FasL ICD; Fas ligand; Receptor-binding FasL ectodomain; sFasL; Soluble Fas ligand; SPA; SPPL2A-processed FasL form; TNFSF6; Tumor necrosis factor ligand superfamily member 6
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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