Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:1000~1:2000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8.
Specificity	p-Smad2/3 (T8) polyclonal antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at Thr8.
Isotype	IgG
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 55,60 kDa
Database Link	UniProt ID P84022/Q15796
Background	Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.
Synonyms	hMAD-2; hMAD-3; hSMAD2; hSMAD3; JV15-2; JV18-1; Mad-related protein 2; Mad3; MADH2; MADH3; MAD homolog 2; MAD homolog 3; MADR2; Mothers against decapentaplegic homolog 2; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 2; Mothers against DPP homolog 3; SMAD2; Smad2; SMAD 2; SMAD 3; SMAD3; Smad3; SMAD family member 2; SMAD family member 3
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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