DARPP32 (PPP1R1B) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:500~1:1000 IHC: 1:50~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide derived from human DARPP-32 around the phosphorylation site of Threonine 75.
Specificity	p-DARPP-32 (T75) polyclonal antibody detects endogenous levels of DARPP-32 protein only when phosphorylated at Thr75
Isotype	IgG
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 32 kDa
Gene Name	protein phosphatase 1 regulatory inhibitor subunit 1B
Database Link	Entrez Gene 84152 Human UniProt ID Q9UD71
Background	Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson’s disease and drug abuse. DARPP-32 (for dopamine and adenosine 3’,5’-monophosphate-regulated phosphoprotein of 32 kDa) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1.
Synonyms	DARPP-32; DARPP32; Dopamine- and cAMP-regulated neuronal phosphoprotein; PPP1R1B; Protein phosphatase 1 regulatory subunit 1B
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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